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The overall objective is to understand the role of bacteria in decomposition of phytoplankton biomass and the importance of this process in the trophic dynamics of Chesapeake Bay. Specific objectives are to analyze biochemically suspended phytodetritus, estimate bacterial production and respiration derived from decomposition of phytodetritus, investigate the utilization of particulate substrates by bacteria in laboratory model systems, and study bacterial production in the mesohaline and oligohaline regions of the Bay.