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By employing oyster larvae and spat as experimental organisms, we propose to develop and test biochemical and molecular procedures for assessing the potential toxicity of selected metals and organic pollutants. Specific objectives include: (i) cloning the cDNAs of hsp genes, (ii) developing quantitative dot blot assays for MT and hsp mRNAs, and (iii) studying the expression of MT and hsp genes and the synthesis of their gene products during various developmental stages in response to induction by metals and organic compounds.