R/CBT-17

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Effects of trace metals and organic pollutants on stress-induced proteins

Principal Investigator:

Thomas T. Chen

Start/End Year:

1991 - 1993

Institution:

Center of Marine Biotechnology, University of Maryland Biotechnology Institute

Co-Principal Investigator:

Guritno Roesijadi, Chesapeake Biological Laboratory, University of Maryland Center for Environmental Science

Topic(s):

Description:

By employing oyster larvae and spat as experimental organisms, we propose to develop and test biochemical and molecular procedures for assessing the potential toxicity of selected metals and organic pollutants. Specific objectives include: (i) cloning the cDNAs of hsp genes, (ii) developing quantitative dot blot assays for MT and hsp mRNAs, and (iii) studying the expression of MT and hsp genes and the synthesis of their gene products during various developmental stages in response to induction by metals and organic compounds.

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