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Specific objectives will be to map the usage patterns of Dimilin in the northeast Chesapeake Bay catchment area, and monitor Dimilin run-off at two field sites. Bioassay results from two crustacean species at these field sites will be compared with bioassay data from a (Dimilin-free) reference site. End points from these and laboratory bioassays will characterize the role of food in Dimilin bioavailability and will form the basis of hazard assessment for this compound's specific mode of action. Chemical application, run-off and toxicity data will be combined as a model risk assessment for non-point source pesticide run-off.