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R/BT-08
Production of recombinant fish gonadotropins (GtHs) using eukaryotic expression systems and development of a striped bass gth immunoassay
Principal Investigator:
Yonathan ZoharStart/End Year:
1998 - 2001Institution:
Center of Marine Biotechnology, University of Maryland Biotechnology InstituteCo-Principal Investigator:
B Weintraub, University of Maryland Biotechnology Institute, Medical Biotechology Center.; M Szkudlinski, University of Maryland Biotechnology Institute, Medical Biotechology CenterTopic(s):
Description:
To produce immunologically and biologically active recombinant striped bass GtH-I and GtH-II, and to develop an enzyme-linked immunosorbant assay (ELISA) for striped bass GtH-I. To use and optimize a novel and generic recombinant DNA approach for expressing biologically and immunologically active gonadotropins of fish, in view of enabling studies on their reproductive endocrinology.
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Featured Fellow
Leone Yisrael is a cephalopod-loving scuba diver, cook, and loves to try new activities. She conducts genetic analysis and fieldwork at the Smithsonian Environmental Research Center through the Coastal Disease Ecology Lab.
Featured Research Project
Developing a habitat model for mysids, an important link in Chesapeake Bay food webs
Mysids are important mesozooplankton prey for many species of fish in Chesapeake Bay and are an important link in transferring energy from lower to upper trophic levels. Mysids also serve as biological vectors for benthic-pelagic coupling due to their diel vertical migration and omnivorous prey-switching behavior, which makes mysids important regulators of food web architecture. Despite their central role in coastal food webs, surprisingly little is known about mysid ecology and dynamics in Chesapeake Bay.
