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The overall objective of this project is to quantify the bioaccumulation and food web dynamics of hydrophobic organic compounds (HOCs: primarily PCBs, chlordanes and DDTs) in Anacostia River via exposure through the water column (e.g. suspension feeders, predators) and the benthic (e.g. deposit feeders, suspension feeders) communities in the sediments of the river. Specific objectives are to determine the contaminant level and season variations in important food sources to benthic and pelagic fish in the river, and determine the inter-relationships among primary producer-consumer-predator using multiple techniques including stable isotopes, gut content analysis, and PCB and chlordane concentration and fingerprinting analysis.