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This proposed research will continue our efforts to 1) characterize the seasonal changes in the composition of dissolved organic nitrogen (DON) and dissolved organic carbon (DOC) along the axis of Chesapeake Bay, both in terms of specific compounds and molecular weight distributions; 2) determine how much DON and DOC is biologically available to bacteria and phytoplankton in short-term assays and mesocosm studies; 3) compare the rates of supply with estimates of cell size and proportion of active cells.