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R/ODR-10
Monoclonal Antibody ELISA Assay for Detection of Perkinsus marinus in Oyster Tissues
Principal Investigator:
Christopher F. DunganStart/End Year:
1997 - 2001Institution:
Maryland Department of Natural ResourcesTopic(s):
Description:
Produce, clone, and cryopreserve, immortal rmurine hybridomos secreting IgG class monoclonal antibodies (MAB) which bind to P marinus cell walls and soluble proteins. Test binding specificities of MABs for P marinus using fluorescence immunoessays of various histological samples containing diverse Perkinsus sp. pathogens, and other possible related protozoans. Optimize MAB utilities as ELISA assay reagents, evaluate clinical performance of MAB diagnostic ELISA assay, and localize subcellular MAB binding epitopes.
Related Publications:
Jordan, SJ; Coakley, JM. 2004. Long-term projections of eastern oyster populations under various management scenarios. Journal of Shellfish Research23(1):63 -72. UM-SG-RS-2004-16.
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Featured Fellow
Leone Yisrael is a cephalopod-loving scuba diver, cook, and loves to try new activities. She conducts genetic analysis and fieldwork at the Smithsonian Environmental Research Center through the Coastal Disease Ecology Lab.
Featured Research Project
Developing a habitat model for mysids, an important link in Chesapeake Bay food webs
Mysids are important mesozooplankton prey for many species of fish in Chesapeake Bay and are an important link in transferring energy from lower to upper trophic levels. Mysids also serve as biological vectors for benthic-pelagic coupling due to their diel vertical migration and omnivorous prey-switching behavior, which makes mysids important regulators of food web architecture. Despite their central role in coastal food webs, surprisingly little is known about mysid ecology and dynamics in Chesapeake Bay.
